What is mammalian expression vector?
Mammalian expression vectors are used to introduce a specific fragment of DNA into mammalian systems for RNA or protein expression. These systems include human and mouse cell lines, and even in vivo in live animals.
How to express gene in mammalian cells?
DNA of interest is first cloned into an appropriate vector and then by transfection, the gene can be inserted into the host for its expression. For expressing hetrologous genes in mammalian cells, usually vectors derived from mammalian viruses are used.
How do you choose a mammalian expression vector?
When you are choosing an expression vector, you’d better keep these things in mind:
- Expression System. You need to choose an expression vector that is specific for the expression system.
- Purification Tag.
- Applications of the Target Protein.
- Strength of the Promoter.
- Selectable Marker.
- Vector Size.
What are characteristics of expression vector?
An expression vector has features that any vector may have, such as an origin of replication, a selectable marker, and a suitable site for the insertion of a gene like the multiple cloning site.
What is an example of cloning vector?
Cloning vectors are used to introduce foreign DNA into host cells, where that DNA can be reproduced (cloned) in large quantities. Examples of cloning vectors are plasmids, cosmids, bacterial artificial chromosomes (BACs), and yeast artificial chromosomes (YACs).
How are expression vectors used in live animals?
Mammalian expression vectors are used to introduce a specific fragment of DNA into mammalian systems for RNA or protein expression. These systems include human and mouse cell lines, and even in vivo in live animals.
Are there any flexible options for mammalian protein expression?
We offer many flexible options for mammalian protein expression, including Invitrogen TOPO and Gateway expression vectors and mammalian cell culture products. When minimizing waste in your lab, every little bit helps.
Which is the best vector for protein expression?
These vectors use the strong CMV/IE enhancer/promoter and an SV40 replication origin to give high levels of protein expression in mammalian cells. Although most pD600s have mammalian selectable markers, we recommend the Bifunctional Leap-In® vectors for generating stable cell lines.
How does Atum’s stable mammalian expression vector work?
ATUM’s stable mammalian vectors are based on our Leap-In transposon technology. Leap-In transposases integrate the entire sequence of the plasmid between the left and right transposon borders into the host genome, as multiple independent copies.
