What is vivaspin?
Vivaspin® centrifugal concentrators are disposable ultrafiltration devices for the concentration of biological samples. The patented vertical membrane design and thin channel filtration chamber (US 5,647,990), minimises membrane fouling and provides high speed concentrations.
What is centrifugal concentrator?
Centrifugal Vacuum Concentrators. CentriVap Centrifugal Vacuum Concentrators use a combination of centrifugal force, vacuum and heat to speed laboratory evaporation of multiple small samples. Sample sizes range from a few microliters up to 25 milliliters.
What is protein concentrator?
Thermo Scientific Pierce concentrators are disposable ultrafiltration centrifugal devices with a polyethersulfone (PES) membrane for the concentration, desalting and buffer exchange of biological samples.
What is PES membrane?
PES is an inherently hydrophilic membrane that wets out quickly and completely resulting in fast filtration with superior flow rates and high throughputs. PES membrane is also extremely low protein binding minimizing the likelihood of target analyte binding.
What is molecular weight cut off membrane?
Molecular weight cut-off or MWCO refers to the lowest molecular weight solute (in daltons) in which 90% of the solute is retained by the membrane, or the molecular weight of the molecule (e.g. globular protein) that is 90% retained by the membrane.
How does a vacuum concentrator work?
In a vacuum concentrator, samples are held in a rotor that spins at 1700 RPM creating a centrifugal force. The centrifugal force prevents liquids from bumping out of the tube when vacuum is applied. Heat is applied indirectly through the walls of the vacuum chamber.
How does a genevac work?
Centrifugal concentrators work by removing solvent under vacuum, so they’re effectively boiling the solvent at a very low temperature. As the pressure in a system decreases, the boiling point of a solvent also decreases, allowing the solvent to boil and turn to a gas at a very low temperature.
How do I choose a protein concentrator?
Select a molecular weight cutoff (MWCO) that is about 1/3 the molecular weight of your protein. There is no advantage to going with a lower MWCO – it just takes longer. Use the maximum g-force that the concentrator is rated for to concentrate the sample in the shortest time.
How does protein concentrator work?
Protein concentrators The most common of these use centrifugation to force the protein solution through a membrane, but others use a pressure differential, either by pumping nitrogen into a sealed vessel above the protein solution or by creating a vacuum on the opposite side of the membrane from the protein solution.
How do you use a concentrator?
Steps to set up an oxygen concentrator Connect the humidification bottle if it is prescribed by the doctor. It is required if the oxygen flow rate is higher than 2-3 litres per minute. Use only distilled or filtered water in the humidification bottle. Attach the oxygen tubing to the humidification bottle or an adaptor.
Is PES membrane hydrophilic?
For General Filtration Applications PES is an inherently hydrophilic membrane that wets out quickly and completely resulting in fast filtration with superior flow rates and high throughputs. PES membrane is also extremely low protein binding minimizing the likelihood of target analyte binding.
What are the features of the vivaspin 20 concentrator?
Vivaspin™ 20 features twin vertical membranes for unparalleled filtration speed and >100-fold concentration. Volume graduation allows to monitor the concentration process and the modified dead stop pocket simplifies pipetting of the final concentrate.
What are the spin columns of vivaspin used for?
Vivaspin protein concentrator spin columns Vivaspin™ ultrafiltration spin columns are designed for fast, non-denaturing concentration and desalting of proteins and other biological samples.
Can you use vivaspin 20 for gas pressure filtration?
When an appropriate centrifuge is unavailable, or for single sample processing, for gas pressure filtration, Vivaspin™ 20 can be filled with up to 15ml and then pressurised for bench top concentration. For even faster processing, gas pressure filtration can be combined with centrifugation.
How does vivaspin centrifugal concentrators minimize solvent fouling?
Vivaspin® Centrifugal Concentrators Centrifugation provides the force to rapidly remove solvents and small molecules through the ultrafiltration membrane. The patented vertical membrane design and thin channel filtration support (US 5,647,990) minimizes filter fouling and allows high speed concentration.
