What is the use of colorimeter in laboratory?
A laboratory colorimeter, also referred to as a digital colorimeter, is an instrument used to measure the absorbance of wavelengths of light at a particular frequency (color) by a sample.
How does a photoelectric colorimeter work?
In a colorimeter, a beam of light with a specific wavelength is passed through a solution via a series of lenses, which navigate the colored light to the measuring device. This analyzes the color compared to an existing standard. A microprocessor then calculates the absorbance or percent transmittance.
Which is the part of photoelectric colorimeter?
A diagram of the basic arrangement of a typical colorimeter is given in Fig 10.12. White light from a tungsten lamp passes through a slit then a condenser lense, to give a parallel beam which falls on the solution under investigation contained in absorption cell or cuvette.
Where is colorimetry used?
Uses of colorimetry Colorimetry is used in chemistry and in other sorts of places such as in industries, colour printing, textile manufacturing, paint manufacturing and in food industries (including the chocolate industry). Colorimetry is also used in aspirin.
What is purpose of colorimeter?
A colorimeter can measure the absorbency of light waves. During colour measurement the change in the intensity of electromagnetic radiation in the visible wavelength region of the spectrum after transmitting or reflecting by an object or solution is measured.
What is the function of colorimetry?
Colorimetry is a scientific technique that is used to determine the concentration of colored compounds in solutions by the application of the Beer–Lambert law, which states that the concentration of a solute is proportional to the absorbance.
What is the aim of colorimetry?
What is meant by photoelectric colorimeter?
In biochemistry, the photoelectric colorimeter is commonly employed for measurement of different substances in the blood like glucose, blood urea, blood enzymes, and others. It is used for measuring the transmittance and absorbance of light passing through a liquid sample.
Which light source is used in colorimeter?
In colorimetry, frequently the entire visible spectrum (white light) is used, and consequently the complementary colour of the one absorbed is observed as transmitted light. If monochromatic light or a narrow band of radiation is used, the instrument is called a spectrophotometer.
What are the advantages of colorimetry?
Advantages of Colorimetry
- It is fast, economical, and has a simple operation of a spectrometer.
- It is a quite fast and convenient method to that of the gravimetric or volumetric processes, and they are easily optimized for automation.
- To handle the Colorimeter, it does not require an experienced person.
What is colorimeter principle?
The working principle of the colorimeter is based on Beer-Lambert’s law which states that the amount of light absorbed by a color solution is directly proportional to the concentration of the solution and the length of a light path through the solution.
Why colorimetric method is important?
Colorimetry, measurement of the wavelength and the intensity of electromagnetic radiation in the visible region of the spectrum. It is used extensively for identification and determination of concentrations of substances that absorb light.
How is the photoelectric colorimeter used in medicine?
Photoelectric colorimeter is a device that is used for the quantitative analysis of different substances like glucose, blood urea, blood enzymes etc. It works on the principle of absorption of light which is explained in detail below. Most of the students learning advanced sciences know about the Beer-Lambert’s Law.
How is colorimeter used in the field of Chemistry?
The field of chemistry is full of devices that are used for different tests and measurements. Operating these devices take some good technique and learning. Colorimeter is one of those devices in the field of chemistry. The light waves of various wavelengths can be absorbed different solutions.
How are light waves absorbed in a colorimeter?
Colorimeter is one of those devices in the field of chemistry. The light waves of various wavelengths can be absorbed different solutions. However, before this device, we didn’t know exactly how to calculate that.
What kind of primaries are used in colorimeter?
Wright used an elaborate optical system to separate three wavelengths, namely 460, 530 and 650 nm, from white light to use as primaries. However, they produce a metameric match, and the results vary from observer to observer. Donaldson (1947)modified the instrument by using six primaries to overcome the problem of metamerism.
