What is the main function of Microhematocrit centrifuge?
Microhematocrit centrifuges are used for determination of volume fractions of erythrocytes (red blood cells) in blood and for separation of micro volumes of blood and solutions. It provides hematocrit values (HCT) for testing in biochemistry, immunity, genetics, blood separation and for general clinical tests.
What is the centrifugation requirement for Microhematocrit determination?
For the microhematocrit method, after filling the blood in a capillary tube, the two ends of the tube (commonly 75 mm long, 1 mm diameter) are sealed with clay sealant or heat. Then, it is centrifuged at a rate of 11000 to 12000 rpm for 4 to 5 min.
How is Microhematocrit done?
Microhematocrit method, n – The determination of the packed cell volume (PCV) using a small quantity of whole blood, a capillary tube, and a high-speed centrifuge. Packed cell volume, PCV, n – The measure of the ratio of the volume occupied by the red blood cells to the volume of whole blood, expressed as a fraction.
What are the different types of centrifuge?
There are different types of centrifuge used for the separation of different molecules, but they all work on the principle of sedimentation….
- Benchtop centrifuge.
- Continuous flow centrifuge.
- Gas centrifuge.
- Hematocrit centrifuge.
- High-speed centrifuge.
- Low-speed centrifuge.
- Microcentrifuge.
What are the uses of centrifuge?
Some common applications for centrifuges are listed below:
- Separation of mixtures with close densities.
- Separate immiscible liquids.
- Sediment suspended solids.
- Separation of blood.
- Separation insoluble particles (e.g. insoluble proteins in a protein solution)
- Isotope Separation.
- Gravity simulation environments for astronauts.
Is 30 PCV normal?
PCV and Hb measurements are considered as haematological indicators for classifying the severity of anaemia, with PCVs in the range of 21-30% and Hb 9.5-13 g/dl being considered as mild anaemia, PCVs of 15-20% and Hb 8-9.5 g/dl as moderate anaemia, and <15% PCV and Hb <8 g/dl as severe anaemia [7]. …
What is the normal range for male and female PCV?
Published data from Europe and North America indicate that for non-iron-deficient adult Caucasian males, the normal mean packed cell volume (PCV) is 0.46 and the 2.5-97.5 percentile interval is 04.0-0.53. Corresponding values for adult Caucasian females are: mean PCV 0.42; 2.5-97.5 percentile interval 0.36-0.48.
What is the normal range for PCV?
| Haematology Normal Adult Reference Ranges | Male | Female |
|---|---|---|
| Platelet Count (PLT) | 150-450 | 150-450 |
| Red Blood Count (RBC) | 4.5-6.5 | 3.8-5.8 |
| Mean Cell Volume (MCV) | 80-100 | 80-100 |
| Packed Cell Volume (PCV)/Haematocrit (HCT) | 0.40-0.52 | 0.37-0.47 |
What does a spun Microhematocrit tube determine?
Microhematocrit. The reference method recommended by NCCLS of determining hematocrit or packed cell volume (PCV) is centrifugation. Method [4]: Hematocrit (PCV) is the measure of the ratio of the volume occupied by the red blood cells to the volume of whole blood.
How do you spin a PCV valve?
Place both tubes in the centrifuge grooves directly opposite one another, this ensures the centrifuge is balanced. Ensure the clay seals are towards the outside. Screw the centrifuge cover into place and close the lid. Spin at 10,000rpm for five minutes (the speed and time may vary on some models).
How are microhematocrit centrifuges used in blood work?
Microhematocrit Centrifuges. By applying centrifugal force, it is possible to separate suspended particles in a fluid or to separate liquids that have different densities. In particular, microhematicrit centrifuges can be used to determine the ratio of the volume of red blood cells to the total volume of blood (hematocrit).
What are the specifications of a heparinized centrifuge?
Heparinized tubes for capillary blood Clay or critoseal Microhematocrit centrifuge Specifications: radius should be greater than 8.0 cm capable of reaching maximum speed in 30 seconds sustains a RCF of 10,000-15,000 x g for five minutes without exceeding a temperature of 45°C Microhematocrit reading device Quality Control
How to check the accuracy of a microhematocrit determination?
A quality control program for microhematocrit determinations should include: Centrifuge calibration: The centrifuge timer should be checked for accuracy and reproducibility with a stopwatch. The centrifuge speed should be checked with a properly calibrated tachometer.
How do you measure hematocrit in capillary tubes?
Place the capillary tubes in the microhematocrit centrifuge with the sealed end toward the periphery. Duplicate tubes should be opposite each other for balance. Centrifuge for five minutes. Using a microhematocrit reading device, determine the hematocrit.
