What is the molecular weight of alpha tubulin?
50 kDa
Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22 nm diameter with a theoretical molecular weight of 50 kDa.
How big is tubulin kDa?
Detects a band of approximately 51 kDa (predicted molecular weight: 50 kDa). Can be blocked with Human beta Tubulin peptide (ab20775).
Why is beta tubulin positive?
Beta tubulins are one of two structural components that form our microtubule network. Since it was discovered that the human brain generates new neurons from stem cell pools, beta tubulin III has been used as a marker of positive neuronal identity in many research studies.
What is vinculin antibody?
vinculin Antibody (H-10) is a mouse monoclonal IgG2a κ, cited in 116 publications, provided at 200 µg/ml. raised against amino acids 1-300 of vinculin of human origin. Anti-vinculin Antibody (H-10) is recommended for detection of vinculin of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA.
How is tubulin formed?
They are formed by the polymerization of a dimer of two globular proteins, alpha and beta tubulin into protofilaments that can then associate laterally to form a hollow tube, the microtubule. The most common form of a microtubule consists of 13 protofilaments in the tubular arrangement.
What is the purpose of vinculin?
Vinculin is a cytoplasmic actin-binding protein enriched in focal adhesions and adherens junctions that is essential for embryonic development. Much is now known regarding the role of vinculin in governing cell–matrix adhesion.
What is the molecular weight of vinculin?
124 kDa
Product Information
| Tested Reactivity | human, mouse, rat, pig |
|---|---|
| Full Name | vinculin |
| Calculated molecular weight | 1133 aa, 124 kDa |
| Observed molecular weight | 117 kDa |
| GenBank accession number | BC039174 |
What is the molecular weight of beta tubulin?
Beta Tubulin Molecular Weight. Each beta tubulin and alpha tubulin has a molecular weight of approximately 50 kiloDaltons. These subunits are slightly acidic with an isoelectric point between 5.2 and 5.8.
What does tubulin stand for in molecular biology?
Tubulin in molecular biology can refer either to the tubulin protein superfamily of globular proteins, or one of the member proteins of that superfamily. α- and β-tubulins polymerize into microtubules, a major component of the eukaryotic cytoskeleton.
How are microtubules assembled from dimers of tubulin?
Microtubules are assembled from dimers of α- and β-tubulin. These subunits are slightly acidic with an isoelectric point between 5.2 and 5.8. Each has a molecular weight of approximately 50 kDa. To form microtubules, the dimers of α- and β-tubulin bind to GTP and assemble onto the (+) ends of microtubules while in the GTP-bound state.
How many tubulins are there in a human cell?
There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules.
