What is Argyrophil reaction?
PRINCIPLE: Argyrophil cells are capable of being impregnated with silver, but need a reducing agent to reduce the silver to a visible metallic silver. While argentaffin cells have both the ability of being impregnated with silver and reducing the silver.
What are Argyrophil cells?
Argyrophil cells are neuroendocrine cells which react with silver stain resulting in brown or black coloration with the help of an external reducer. Argyrophil cells occur rarely in the normal human uterine cervix but are found in a variety of invasive tumors arising in the cervix.
What is the function of argentaffin cells?
The argentaffin cells are granular cells that secretes serotonin which is responsible for the peristaltic movement of the muscles of the digestive tract.
Where are argentaffin cells present?
Argentaffin cell, one of the round or partly flattened cells occurring in the lining tissue of the digestive tract and containing granules thought to be of secretory function. These epithelial cells, though common throughout the digestive tract, are most concentrated in the small intestine and appendix.
What is silver staining used for?
Silver staining is an excellent technique for detecting proteins which are separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis due to its efficiency of detecting proteins present in nanograms.
What does reticulin stain?
Reticulin Stain. The reticulin stain is extensively used in the histopathology laboratory for staining liver specimens, but can also be used to identify fibrosis in bone marrow core biopsy specimens.
Are Paneth cells in the colon?
While Paneth cells have occasionally also been found patchily dispersed in the stomach and colon, this is generally associated with mucosal inflammation as opposed to homeostasis (4). Although Paneth cells were first discovered and described in humans, they are not specific to humans.
Which gland is present in stomach?
Gastric gland, any of the branched tubules in the inner lining of the stomach that secrete gastric juice and protective mucus.
Is silver stain quantitative?
Silver staining has a narrow linear dynamic range (the range at which the level of staining is linear to the concentration), making it less suitable for quantification.
How sensitive is silver staining?
Silver staining Kits with optimized protocols are robust and easy to use, detecting less than 0.5 ng of protein in typical gels. Silver stains use either glutaraldehyde or formaldehyde as the enhancer.
