What are the different types of columns used in HPLC?
Different Types of HPLC Columns Used in Analysis
- Normal Phase Columns.
- Reverse Phase Columns.
- Ion Exchange Columns.
- Size Exclusion Columns.
What is the difference between C8 and C18 HPLC columns?
C18 has 18 carbon atoms while C8 has only 8 carbon atoms. C18 has a longer carbon chain, but C8 has a shorter one. C18 has higher retention while C8 has shorter retention. C18 has higher hydrophobicity, but C8 has a lower hydrophobicity.
What are C18 columns?
C18 columns are HPLC (high performance liquid chromatography) columns that use a C18 substance as the stationary phase. C18 HPLC columns are used in environmental sciences and chemical analysis, as well as industries such as pharmaceutical and environmental sciences, to analyze individual parts of chemical mixtures.
What’s the difference between pillars and columns?
A pillar is a vertical support member and may be constructed as a single piece of timber, concrete or steel, or built up out of bricks, blocks and so on. However, whereas a pillar does not necessarily have a load-bearing function, a column is a vertical structural member that is intended to transfer a compressive load.
What are square columns called?
pilaster — A squared column (i.e., a pier) protruding from a wall. engaged column — A round column protruding from a wall like a pilaster. post or stake or pole. pier — A squared column. buttress.
What is SST in HPLC?
System suitability test (SST) is a test to determine the suitability and effectiveness of chromatographic system prior to use. These mixtures are used to establish characteristic chromatographic parameters, such as the number of effective theoretical plates, resolution, asymmetry, detection limit and selectivity.
What are the different types of Sielc columns?
Two complimentary columns, Obelisc R and Obelisc N, based on Liquid Separation Cell ( LiSC ™) technology, offer a new approach to separate a variety of small molecules.
What kind of hardware is Sielc for HPLC?
HPLC Hardware SIELC Technologies is offering a patented design of capillary, analytical, and preparative hardware for HPLC. Unified cylindrical shape of the columns is a signature hardware style developed and produced by SIELC. Superior performance, distinguished look, and reduced hardware costs are just a few benefits of the new design.
Which is Sielc primesep guard columns do you need?
A wide range of HPLC columns with id’s from 0.5 mm to 22 mm are available. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers.
Can a column be used in a HPLC heater?
The column hardware was designed specifically to be used in any HPLC instrument. It can be installed in most column heaters as well. This column is available in two hardware versions: traditional two ended and new SIELC hardware with only one end.
