What type of cancer is MDA-MB-231?
The MDA-MB-231 cell line is an epithelial, human breast cancer cell line that was established from a pleural effusion of a 51-year-old caucasian female with a metastatic mammary adenocarcinoma1 and is one of the most commonly used breast cancer cell lines in medical research laboratories.
What is MDA MB 468 cell line?
From Wikipedia, the free encyclopedia. MDA-MB-468 is a cell line that was isolated from a 51-year-old female human in 1977, and is commonly used in breast cancer research.
How do you freeze MDA-MB-231 cells?
Prepare cryopreservation medium by adding DMSO to cold complete growth medium (L- 15 + 10 % (v/v) FBS) at a final concentration of 5 % (v/v) DMSO. Place cryopreservation medium on ice until ready to use.
Is MCF7 triple negative?
MCF7 and MDA-MB-231 cells represent a striking example in that they are both invasive ductal/breast carcinoma cells, yet they have many phenotypic/genotypic differences: MCF7 are hormone dependent (both estrogen and progesterone receptor positive—ER and PR), while MDA-MB-231 are triple negative.
How long does it take MDA-MB-231 cells to adhere?
Do not change the media or split cells before they are about 60% confluent (which can take 5-7 days for MDA-MB-231, depending on how many survive thawing), unless there are a lot of dead cells floating.
How do you prepare freezing media?
Prepare pre-warmed medium in advance. Remove cryovials from liquid nitrogen and immediately place in 37°C water bath and quickly shake until about 80% has thawed. This should not take more than a minute. Quickly pipette out into a flask, add the appropriate amount of medium and place in incubator.
How do you freeze Hela cells?
Procedure
- trypsinize 10 flasks with 2ml Tryp.
- suspend cells in some medium (~ 8ml for 3 flasks)
- pool the cell suspensions in a 50ml centrifuge tube.
- centrifuge 5min/1500 rpm.
- remove the supernantant.
- resuspend the cell pellet in 20ml freezing medium (regular growth medium + 5% sterile DMSO)
- aliquot in 20 x 1ml Cryo tubes.
What is triple-negative?
Triple-negative breast cancer (TNBC) accounts for about 10-15% of all breast cancers. The term triple-negative breast cancer refers to the fact that the cancer cells don’t have estrogen or progesterone receptors and also don’t make too much of the protein called HER2. (The cells test “negative” on all 3 tests.)
What does HER2-positive mean?
HER2 is a growth-promoting protein on the outside of all breast cells. Breast cancer cells with higher than normal levels of HER2 are called HER2-positive. These cancers tend to grow and spread faster than other breast cancers, but are much more likely to respond to treatment with drugs that target the HER2 protein.
What is MCF7 cell line?
MCF-7 is a human breast cancer cell line with estrogen, progesterone and glucocorticoid receptors [26]. It is derived from the pleural effusion of a 69-year-old Caucasian metastatic breast cancer (adenocarcinoma) in 1970 by Dr Soule of the Michigan Cancer Foundation, Detroit, MI [27].
